Next Generation Sequencing



The clinical gene amplification test laboratory passed the inspection and acceptance of experts from the Health Inspection Center to conduct human leukocyte antigen gene (HLA) typing tests. Relying on the Zhongguancun Open Laboratory, the research, and transformation of disease susceptibility gene detection, early disease screening, differential diagnosis, medication guidance, prognostic evaluation and recurrence monitoring of a series of individualized treatment testing technology. The technology involves the extraction of genomic DNA, extraction of plasmid DNA, RNA extraction, detection of mRNA expression levels, gene mutation, SNP detection, and whole-genome sequencing.

SNP/Mutation Detection Analysis The complete solution provides a complete solution for SNP analysis customers: test process design, primer design and synthesis, PCR amplification and purification, sequencing, and data analysis. 
Flexible experimental protocol 
      We can customize the experimental protocol based on the size of your sample and the length of your study area. SNP analysis uses the ABI 3730XL DNA Analyzer to sequence the sample genes. The applicable sample size can range from tens to thousands, and the SNP search area can range from 200 bases to several thousand bases. 
The result is highly accurate 
      To ensure accuracy and detection sensitivity, we only retain the high-quality region of the sequence for analysis. Dedicated analysis software improves sequence analysis efficiency and guarantees detection of all diversity sites, including heterozygous sites. 


Segoe UI Symbol"">★ Service Content 


1. Known SNPs/mutation sites analysis and detection 
      Sequencing of known SNPs must be used for sequencing, which is widely used for genetic diversity analysis or gene diagnosis of known pathogenic genes. 


2. Finding Unknown SNPs/Mutation Sites 


1. Exon sequencing 
      exons contain the information needed to synthesize proteins. Sequencing the candidate exons of the disease can reveal new SNPs/mutations in the gene. This will lay an important foundation for studying the relevance of disease and candidate genes. 


2. Whole gene sequencing 
      If the SNP site is located in the gene regulatory region, the SNP site in the intron and the intergenic region may have an influence on the expression level of the gene, thus affecting the protein expression and related traits. Therefore, it is also very necessary to perform a new SNP/mutation site search within the whole genome. 


Segoe UI Symbol"">★ The information you need to provide: 
      Required information such as gene information to be detected, SNP locus information, and reference sequences.
      Genomic DNA or -20 degrees preserved blood and tissues. 


Segoe UI Symbol"">★ SNP service commitment: 
      We provide you with fast and high-quality test result data, including sequencing peak maps, SNP analysis reports, and complete experimental reports.


 1.1.1                Sequencing Service

 

We offered DNA sequencing services to researchers around the world. Our DNA sequencing services include a broad range of offerings that meet your needs, whether you are engaged in basic genomics research or in biological drug development and manufacturing. Sequencing reactions are performed using Applied Biosystems BigDye V3.1 Chemistry and analyzed on ABI 3730XL DNA Analyzers.

DNA Sequencing by Sample Format

 

 

1.1.1.1             Next Generation Sequencing / Whole-genome Sequencing

 

Service

Description

Run-only Sequencing

Customers send sequencing plates that are ready to be loaded to on the ABI 3730XL.

Fast Turn-around Sequencing

Customers send DNA samples (in tubes, strips, or plates) that are ready to be sequenced with either our in-house DNA sequencing primers or specific sequencing primers provided by the customer. Over-night data delivery.

High-throughput Sequencing

Customers send DNA samples (in 96-well plates) that are ready to be sequenced using a single DNA sequencing primer.

Custom Sequencing

Customers send samples of any type (tissue, blood, glycerol stock, etc). DNA or RNA will be prepared, and optional PCRs will be performed, followed by sequencing with primers designed by Avance Biosciences.