CAR-T immune cell therapy


1.1.1          CAR-T immune cell therapy

 

 

 

Targeting genes

The scope of application

ScFv source

form

Types of

Remarks

generation

Second generation

Three generations

Four generations

GFP label

CD19

B-cell lymphoma

Mouse

Lentivirus

Optional

1. Provides a stable cell line for overexpression of oncogenes and serves as a positive control for CAR-T functional validation; 
2. Provides construction services for various lentivirus-expressing vectors targeting multiple tumors

CD22

B-cell lymphoma

Mouse

Lentivirus

Optional

GD2

Neuroblastoma

Mouse

Lentivirus

Optional

PSCA

Pancreatic cancer

Mouse

Lentivirus

Optional

PMSA

Prostate cancer

Mouse

Lentivirus

Optional

CEA

Lung cancer

Mouse

Lentivirus

Optional

HER2

Breast cancer, lung cancer, pancreatic cancer and the like

Mouse

Lentivirus

Optional

EGFR

Broad spectrum

Mouse

Lentivirus

Optional

CD3

Acute lymphoblastic leukemia

Mouse

Lentivirus

Optional

HER3 / EGFR

Colorectal cancer, head and neck cancer

Mouse

Lentivirus

Optional

TNF

Rheumatoid arthritis

Mouse

Lentivirus

Optional

HER2, CD3, FcR

Solid tumor

Mouse

Lentivirus

Optional

CD123, CD3

Acute myeloid leukemia

Mouse

Lentivirus

Optional

HER2, HER3

Bone cancer

Mouse

Lentivirus

Optional

 

Chimeric Antigen expression vector construction services, lentivirus packaging

 

According to the specific antigen provided by the customer or the sequence of the CAR

gene, a lentiviral expression vector


 

Lentiviral packaging and determination of virus Concentration

 

based on the lentiviral expression vector provided by the customer, the lentivirus is packaged and tested in conjunction with the flow cytometry and T cell infection results to provide a final lentivirus concentration

 

The initial offer of CAR-T customized service

Experimental steps

Initial offer

The time required

Construction of vectors (including primer design, gene synthesis, specific antigen recognition design, construction of several different lentiviral expression vectors and sequencing validation, etc.)

Probably the price of 50,000-100,000 yuan (5-10 million) 
according to a variety of reagents, vectors, primers, sequencing and other prices to make the appropriate adjustments

2-3 months (Gene synthesis takes 3 weeks)

Lentiviral packaging, infection (acquisition of virions, determination of titer)

about the price at about 20,000 yuan (20,000 yuan)

1-2 months or so

Lentivirus transduction of target cells and in vitro expression detection, detection of various cytokines and killing experiments

Combined with the type of infected cells, the detection of antibodies and the price of various kits, a rough estimation of about 200,000-300,000 yuan (200,000-300,000 yuan)

3-6 months

 

 

Vector Library

Have dozens of commonly used vector for you to choose, to ensure consistency with the official sequence provided

Product number

product name

Carrier type

Price (RMB)

JM-V-001

pLVX-IRES-ZsGreen1

Lentiviral vector

¥ 1,200.00

JM-V-002

PLVX-1st CAR-IRES-ZsGreen1

Lentiviral vector

¥ 50,000.00

JM-V-003

PLVX-1stCAR

Lentiviral vector

¥ 50,000.00

JM-V-004

pCDH-CMV-MCS-EF1-copGFP

Lentiviral vector

¥ 1,200.00

JM-V-005

pCDH-CMV-1st CAR-EF1-copGFP

Lentiviral vector

¥ 50,000.00

JM-V-006

PCDH-CMV-1st CAR

Lentiviral vector

¥ 50,000.00

JM-V-007

PSMPUW-IRES-GFP

Lentiviral vector

¥ 1,000.00

JM-V-008

PSMPUW-1stCAR -IRES-GFP

Lentiviral vector

¥ 50,000.00

JM-V-009

PSMPUW-1stCAR

Lentiviral vector

¥ 50,000.00

JM-V-010

PSMPUW-Lacz

Lentiviral vector

¥ 1,000.00

JM-V-011

PRSV-Rev

Lentiviral packaging plasmid

¥ 1,000.00

JM-V-012

PRSV-VSVG

Lentiviral packaging plasmid

¥ 1,000.00

JM-V-013

Pcgpv

Lentiviral packaging plasmid

¥ 1,000.00

JM-V-014

PUCK-1stCAR

only to save the purpose of the gene

¥ 50,000.00

JM-V-030

PCAMBIA1301

Plant expression vector

¥ 1,000.00

JM-V-031

PCAMBIA3300

Plant binary expression vector

¥ 1,000.00

JM-V-032

PTFCM

Agrobacterium-mediated transformation vector

¥ 1,000.00

JM-V-033

PSKH

Intermediate transformation vector

¥ 1,000.00

JM-V-034

PCB1004

Intermediate transformation vector

¥ 1,000.00

JM-V-035

pBluescriptⅡKS1004

Intermediate vector for transforming vector

¥ 1,000.00

JM-V-036

pBluescript SK +

Prokaryotic expression vector, cloning vector

¥ 1,000.00

JM-V-037

PGEM-7Zf (+/-)

E. coli cloning vector

¥ 1,000.00

JM-V-038

PT

Intermediate vector for transforming vector

¥ 1,000.00

JM-V-039

KN1

Intermediate vector for transforming vector

¥ 1,000.00

JM-V-040

pCIT

RNAi expression vector

¥ 1,000.00

JM-V-041

PIT

RNAi expression vector

¥ 1,000.00

JM-V-042

PCAMBIA3300IT

RNAi transformation vector

¥ 1,000.00

JM-V-043

PCAMBIA3300IT-neo

RNAi transformation vector

¥ 1,000.00

JM-V-050

PGBKT7

Yeast two-hybrid system

¥ 1,000.00

JM-V-051

PGBKT7-lam

Yeast two-hybrid system

¥ 1,000.00

JM-V-052

PGBKT7-53

Yeast two-hybrid system

¥ 1,000.00

JM-V-053

PGBT9

Yeast two-hybrid system

¥ 1,000.00

JM-V-054

PGADT7

Yeast two-hybrid system

¥ 1,000.00

JM-V-055

PGADT7-RecT

Yeast two-hybrid system

¥ 1,000.00

JM-V-056

PB42AD

Yeast two-hybrid system

¥ 1,000.00

JM-V-057

PlexA

Yeast two-hybrid system

¥ 1,000.00


  

 
 
CAR-T immune cell therapy_application_Application_Jing-Meng Stem Cell

CAR-T immune cell therapy


1.1.1          CAR-T immune cell therapy

 

 

 

Targeting genes

The scope of application

ScFv source

form

Types of

Remarks

generation

Second generation

Three generations

Four generations

GFP label

CD19

B-cell lymphoma

Mouse

Lentivirus

Optional

1. Provides a stable cell line for overexpression of oncogenes and serves as a positive control for CAR-T functional validation; 
2. Provides construction services for various lentivirus-expressing vectors targeting multiple tumors

CD22

B-cell lymphoma

Mouse

Lentivirus

Optional

GD2

Neuroblastoma

Mouse

Lentivirus

Optional

PSCA

Pancreatic cancer

Mouse

Lentivirus

Optional

PMSA

Prostate cancer

Mouse

Lentivirus

Optional

CEA

Lung cancer

Mouse

Lentivirus

Optional

HER2

Breast cancer, lung cancer, pancreatic cancer and the like

Mouse

Lentivirus

Optional

EGFR

Broad spectrum

Mouse

Lentivirus

Optional

CD3

Acute lymphoblastic leukemia

Mouse

Lentivirus

Optional

HER3 / EGFR

Colorectal cancer, head and neck cancer

Mouse

Lentivirus

Optional

TNF

Rheumatoid arthritis

Mouse

Lentivirus

Optional

HER2, CD3, FcR

Solid tumor

Mouse

Lentivirus

Optional

CD123, CD3

Acute myeloid leukemia

Mouse

Lentivirus

Optional

HER2, HER3

Bone cancer

Mouse

Lentivirus

Optional

 

Chimeric Antigen expression vector construction services, lentivirus packaging

 

According to the specific antigen provided by the customer or the sequence of the CAR

gene, a lentiviral expression vector


 

Lentiviral packaging and determination of virus Concentration

 

based on the lentiviral expression vector provided by the customer, the lentivirus is packaged and tested in conjunction with the flow cytometry and T cell infection results to provide a final lentivirus concentration

 

The initial offer of CAR-T customized service

Experimental steps

Initial offer

The time required

Construction of vectors (including primer design, gene synthesis, specific antigen recognition design, construction of several different lentiviral expression vectors and sequencing validation, etc.)

Probably the price of 50,000-100,000 yuan (5-10 million) 
according to a variety of reagents, vectors, primers, sequencing and other prices to make the appropriate adjustments

2-3 months (Gene synthesis takes 3 weeks)

Lentiviral packaging, infection (acquisition of virions, determination of titer)

about the price at about 20,000 yuan (20,000 yuan)

1-2 months or so

Lentivirus transduction of target cells and in vitro expression detection, detection of various cytokines and killing experiments

Combined with the type of infected cells, the detection of antibodies and the price of various kits, a rough estimation of about 200,000-300,000 yuan (200,000-300,000 yuan)

3-6 months

 

 

Vector Library

Have dozens of commonly used vector for you to choose, to ensure consistency with the official sequence provided

Product number

product name

Carrier type

Price (RMB)

JM-V-001

pLVX-IRES-ZsGreen1

Lentiviral vector

¥ 1,200.00

JM-V-002

PLVX-1st CAR-IRES-ZsGreen1

Lentiviral vector

¥ 50,000.00

JM-V-003

PLVX-1stCAR

Lentiviral vector

¥ 50,000.00

JM-V-004

pCDH-CMV-MCS-EF1-copGFP

Lentiviral vector

¥ 1,200.00

JM-V-005

pCDH-CMV-1st CAR-EF1-copGFP

Lentiviral vector

¥ 50,000.00

JM-V-006

PCDH-CMV-1st CAR

Lentiviral vector

¥ 50,000.00

JM-V-007

PSMPUW-IRES-GFP

Lentiviral vector

¥ 1,000.00

JM-V-008

PSMPUW-1stCAR -IRES-GFP

Lentiviral vector

¥ 50,000.00

JM-V-009

PSMPUW-1stCAR

Lentiviral vector

¥ 50,000.00

JM-V-010

PSMPUW-Lacz

Lentiviral vector

¥ 1,000.00

JM-V-011

PRSV-Rev

Lentiviral packaging plasmid

¥ 1,000.00

JM-V-012

PRSV-VSVG

Lentiviral packaging plasmid

¥ 1,000.00

JM-V-013

Pcgpv

Lentiviral packaging plasmid

¥ 1,000.00

JM-V-014

PUCK-1stCAR

only to save the purpose of the gene

¥ 50,000.00

JM-V-030

PCAMBIA1301

Plant expression vector

¥ 1,000.00

JM-V-031

PCAMBIA3300

Plant binary expression vector

¥ 1,000.00

JM-V-032

PTFCM

Agrobacterium-mediated transformation vector

¥ 1,000.00

JM-V-033

PSKH

Intermediate transformation vector

¥ 1,000.00

JM-V-034

PCB1004

Intermediate transformation vector

¥ 1,000.00

JM-V-035

pBluescriptⅡKS1004

Intermediate vector for transforming vector

¥ 1,000.00

JM-V-036

pBluescript SK +

Prokaryotic expression vector, cloning vector

¥ 1,000.00

JM-V-037

PGEM-7Zf (+/-)

E. coli cloning vector

¥ 1,000.00

JM-V-038

PT

Intermediate vector for transforming vector

¥ 1,000.00

JM-V-039

KN1

Intermediate vector for transforming vector

¥ 1,000.00

JM-V-040

pCIT

RNAi expression vector

¥ 1,000.00

JM-V-041

PIT

RNAi expression vector

¥ 1,000.00

JM-V-042

PCAMBIA3300IT

RNAi transformation vector

¥ 1,000.00

JM-V-043

PCAMBIA3300IT-neo

RNAi transformation vector

¥ 1,000.00

JM-V-050

PGBKT7

Yeast two-hybrid system

¥ 1,000.00

JM-V-051

PGBKT7-lam

Yeast two-hybrid system

¥ 1,000.00

JM-V-052

PGBKT7-53

Yeast two-hybrid system

¥ 1,000.00

JM-V-053

PGBT9

Yeast two-hybrid system

¥ 1,000.00

JM-V-054

PGADT7

Yeast two-hybrid system

¥ 1,000.00

JM-V-055

PGADT7-RecT

Yeast two-hybrid system

¥ 1,000.00

JM-V-056

PB42AD

Yeast two-hybrid system

¥ 1,000.00

JM-V-057

PlexA

Yeast two-hybrid system

¥ 1,000.00